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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants were being noted but detailed nucleotide sequence data just isn't yet offered.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded through the antibody.
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This tutorial outlines even more achievable optimization of mobile and immunoassay parameters to ensure the very best success are...
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This tutorial outlines further possible optimization of cellular and immunoassay parameters to ensure the absolute best final results are...
Antibodies certain to the blocking peptide not bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody versus the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose associates are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins exhibit distinctive expression and degradation styles which add to the temporal coordination of each and every mitotic party. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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